Ferret VAChT-SAP treatment disrupts spontaneous retinal activity during P15 to P25. (A) Raster plots from MEA recordings depict abnormal spontaneous retinal activity in Ferret VAChT-SAP-treated retinae compared to saline controls. (B) Correlation index analysis of RGC output shows that correlations are reduced at all inter-pair intervals in Ferret VAChT-SAP versus saline groups. (C,D) Ferret VAChT-SAP reduces overall activity levels (C) and overall RGC burst frequency (D) compared to saline controls. (E,F) Treatment with Ferret VAChT-SAP increases RGC burst duration (E) and decreases the number of spikes/burst (F). Panels (B-F) reflect means ± SEMs for grouped data from recordings at P15 (n = 2), P20 (n = 2), and P25 (n = 2). Statistics reflect two-tailed P-values resulting from independent two-sample t-tests.