Effects of Ferret VAChT-SAP treatment on other retinal neuron types. (A,B) Treatment with Ferret VAChT-SAP leads to ablation of calbindin(+) amacrine cells and horizontal cells. (C,D) Calretinin(+) RGCs and HCs are also ablated by immunotoxin treatment. (E,F) Recoverin(+) photoreceptors appear qualitatively similar in both groups, but the retinal sheet is thinner in immunotoxin-treated retinae compared to controls. Data reflect means ± SEMs. Statistics reflect two-tailed P-values calculated from independent two sample t-tests. 'Merge' panels are counterstained with DAPI to reveal cytoarchitecture. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PRL, photoreceptor layer. Scale bars: 100 μm.