Figure 4

Increased apoptosis in the OE of Insm1^-/-embryos does not precede the decrease in nascent neurons. (A-F) Immunohistochemistry for activated cleaved caspase 3 (ACC3), a marker of apoptotic cells, at selected stages. ACC3+ staining is indicated by arrowheads. (G) At E11.5 (and compare (A) and (B)) and E12.5, there is no significant difference in ACC3+ cells (n = 3 embryo pairs, P = 0.63). By E14.5 (compare (C) and (D)), more ACC3+ cells are detected in Insm1^-/-mice than in their paired Insm1^+/+littermates (n = 3 embryo pairs, P < 0.05), an increase that persists at E18.5 (compare (E) and (F); n = 3 embryo pairs, P = 0.11). Data in (G) are presented as mean values ± standard error of the mean (SEM). *P ≤ 0.05. Scale bars: 100 μm.