The injection of lentiviral vectors in the SVZ of organotypic slice cultures gives rise to GFP+migrating neuroblasts in the RMS and labeled neurons in all OB layers. (A) Photomicrograph of a cultured mouse forebrain sagittal section containing the SVZ-RMS pathway, showing the viral injection site (IS) in the SVZ at 2 days post-injection (dpi). Scale bar: 800 μm. Inset. Approximate location of the image shown in (B). (B) Detail of the IS displayed in (A) at higher magnification. Scale bar: 200 μm. (C) Photomicrograph showing GFP+ cells migrating in the RMS at 5 dpi. Scale bar: 100 μm. (D) Photomicrograph of the entire OB showing the arrival of GFP+ cells at 5 dpi. Scale bar: 500 μm. The outline represents the approximate location of the magnification in (E). (E) Photomicrograph of the area outlined in (D), magnified to show GFP+ cells scattered throughout all OB layers. The same slice culture used in (D) is shown at 11 dpi. Scale bar: 200 μm.