Summary of BrZ3 outgrowth timing phenotype. Timeline of premature outgrowth in dbd[+BrZ3] in comparison to controls, in hours APF. Cartoons from representative in vivo z-projections. Colored bars show event times from time-lapse movies (mean ± standard deviation). Control dbd growth cones oriented to the posterior at 33 ± 3.2 h APF (n = 7), soma migration stopped at 36 ± 2 h APF (n = 6), primary dendrite elongated to twice the length of the initial growth cone by 37 ± 4 h APF (n = 4), and the posterior 'pull' indicated dendrite reached the muscle edge at 42 ± 2 h APF (n = 4). The dbd[+BrZ3] growth cone oriented posteriorly by 27 ± 3 h APF (n = 7), migration stopped at 32 ± 3 h APF (n = 11), dendrite reached twice the growth cone length at 29 ± 3 h APF (n = 9), and bent at the muscle edge at 36 ± 5 h APF (n = 8). The black bar denotes the window of BrZ3 action from temperature shift experiments, adjusted for time required to express and remove BrZ3 (Figure 6). The bar extends from the 50% point along the curve for percentage of ectopic arbors after delayed BrZ3 onset, to the 50% point along the curve for percentage of ectopic arbors in BrZ3 offset experiments, with grey shading extending to the 50% point on the curve for full ectopic comb arbor.