Centriole localization is not consistent with a role in microtubule organization in Drosophila neurons. (A) The centriole does not occupy a stereotypic position in developing aCC and RP2 motor neurons. Stage 12 embryos expressed GFP-Fzr in all cells, and aCC and RP2 were labeled with tau-LacZ reporter expression. GFP puncta within neurons are indicated with arrows. (B) Centriole localization in mature neurons is variable. Live imaging of da neurons of larvae expressing red fluorescent protein (RFP)-Fzr (arrows) and tau-GFP was performed. Z-projections are shown. An example of quadrant segregation is shown at the top right. Localization of 21 Fzr puncta within cell bodies was analyzed and quantified.