A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire

Table 3 Enriched Gene Ontology categories from functional annotation clustering

Functional annotation	Enrichment factor
PDZ domain-containing proteins	1.7
Aging/lifespan	1.65
Cell migration/motility	1.65
Nucleoside/nucleotide metabolism	1.55
Reactive oxygen response; oxidative stress	1.27
BESS/MADF chromatin modifying factors	1.24
Programmed cell death	1.24
Amino acid metabolism	1.22
LRR-containing proteins	1.2
GST activity	1.18
Cell-cell junctions/apicobasal polarity	1.13
Malate metabolism	1.01
Other notable annotation clusters
Embryonic morphogenesis	0.82
Chemosensory behavior/olfactory learning	0.8
Hemopoiesis/hemocyte migration	0.78
Synaptogenesis/synapse organization	0.77
Post-embryonic development/imaginal disc	0.7
Gametogenesis	0.54
Spermatogenesis	0.3
Oogenesis	0.26
Ig-like domain-containing	0.4
Axonogenesis/neuron morphogenesis	0.28
RNPs	0.27

Gene Ontology analysis with DAVID [37] was used to identify processes and properties likely to be associated with lola function, based on enrichment in the collection of lola-sensitive genes. Putatively enriched annotation terms and their enrichment factors are shown, derived by functional annotation clustering performed at high stringency setting. Clusters with enrichment factors ≥1.3 are supported at P < 0.05; lower enrichment scores have proportionately lower statistical support but nonetheless are enriched in the dataset relative to background. The background set for calculating enrichment factors consisted of that subset of features on the microarray that passed the pre-processing steps and entered our statistical analysis (10, 376 features), and that could be assigned unambiguously to a single Flybase gene ID (9772 Flybase-defined genes). Retrotransposons were excluded from this analysis.

ISSN: 1749-8104