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Table 1 Quantitative RT-PCR validation of array results

From: A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire

Gene Clone ID Array fold-change (log2) qRT-PCR fold-change (log2) qRT-PCR P-value
spire GH13327 1.24 1.54 2.5 E-5
  SD10157 0.97   
neural lazarillo GH12581 -0.56 -2.40 9.3 E-5
genderblind/CG6070 GH08870 0.29 0.27 0.016
charybde LD32080 0.57 0.33 0.036
  GH09771 0.59   
  LD22381 0.67   
walrus GH09945 0.46 0.27 0.039
  LD07532 0.44   
centaurin gamma 1a GM01069 -0.95 -0.13 0.25
blistery SD01953 -0.57 0.22 0.34
bekka RE14959 -0.47 -0.46 0.61
  RE14259 -0.52   
Kr target @ 95D SD03976 -0.76 0.03 0.79
Target of rapamycin SD02269 -0.33 0.33 0.95
  1. Total RNA was collected from 10 to 12 hour wild type or lola mutant embryos, just as for microarray analysis, and analyzed by Taqman RT-PCR to quantify expression of ten genes that showed altered transcript level in expression arrays. A positive fold change in the table corresponds to increased accumulation of that RNA in the mutant.