A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire

Table 1 Quantitative RT-PCR validation of array results

Gene	Clone ID	Array fold-change (log2)	qRT-PCR fold-change (log2)	qRT-PCR P-value
spire	GH13327	1.24	1.54	2.5 E-5
	SD10157	0.97
neural lazarillo	GH12581	-0.56	-2.40	9.3 E-5
genderblind/CG6070	GH08870	0.29	0.27	0.016
charybde	LD32080	0.57	0.33	0.036
	GH09771	0.59
	LD22381	0.67
walrus	GH09945	0.46	0.27	0.039
	LD07532	0.44
centaurin gamma 1a	GM01069	-0.95	-0.13	0.25
blistery	SD01953	-0.57	0.22	0.34
bekka	RE14959	-0.47	-0.46	0.61
	RE14259	-0.52
Kr target @ 95D	SD03976	-0.76	0.03	0.79
Target of rapamycin	SD02269	-0.33	0.33	0.95

Total RNA was collected from 10 to 12 hour wild type or lola mutant embryos, just as for microarray analysis, and analyzed by Taqman RT-PCR to quantify expression of ten genes that showed altered transcript level in expression arrays. A positive fold change in the table corresponds to increased accumulation of that RNA in the mutant.

ISSN: 1749-8104