Validation of lola null data with an independent microarray experiment. Graphical representation of an experiment assessing the validity of the lola null mutant results by comparison to a dataset derived from an independent microarray experiment. Expression microarrays were performed comparing RNA from embryos bearing the lola single-isoform mutation lolaORC4 to wild-type controls . The 597 features that were altered in accumulation in lola null mutant embryos were assessed for altered expression in lolaORC4: 204 of the 597 were also found to be altered in the single-isoform mutant (false discovery rate = 0.01; grey star). In contrast, in 500 trials of a simulation in which 597 features were selected at random from the lolaORC4 data set (10, 376 features total) and analyzed for change in mutant versus wild type, the median number of features with altered expression in the single-isoform mutant was 18/597 (maximum 38; presented as a histogram of number of loci with altered expression versus number of occurrences of that result).