Figure 8

BMP7 but not BMP6 stimulates Akt phosphorylation in dI neurons. Whole cell lysates of dissociated dI neurons with or without BMP7 or BMP6, at 0.01 and 50 ng/ml for 30 minutes, probed on western blots with α-pAkt as in Figure 7A. Percentage of control for each condition relative to baseline pAkt levels (mean ± SEM): at 0.01 ng/ml, BMP7 = 153 ± 0.5%, and BMP6 = 82 ± 11% (n = 2); at 50 ng/ml, BMP7 = 145 ± 12%, and BMP6 = 105 ± 8% (n = 5). Student's t-tests: pAkt in dI neuron cultures treated with 0.01 or 50 ng/ml BMP7 was significantly different from control pAkt (**P < 0.01, ***P < 0.001). pAkt in cultures treated with 50 ng/ml BMP6 did not differ from control (P = 0.5628).