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Figure 7 | Neural Development

Figure 7

From: Basal progenitor cells in the embryonic mouse thalamus - their molecular characterization and the role of neurogenins and Pax6

Figure 7

Co-expression of various transcription factors with EdU at different pulse durations. Confocal images of E11.5 frontal sections showing double staining of various transcription factors and EdU pulsed for different durations of time. Midline is to the left. (A-D) NeuroD1 is co-localized with EdU in basal locations at 0.5 hours, 2 hours, 4 hours and 8 hours after EdU labeling (arrowheads). (E-H) Lhx2/9 shows co-localization with EdU mostly at lateral locations with 0.5-hour, 2-hour and 4-hour pulses (arrowheads), but with an 8-hour pulse, scattered EdU-positive cells in the VZ as well as in far lateral locations show co-localization. (I-L) With a 0.5-hour pulse, Neurog1 is co-expressed with EdU near the lateral margin of the VZ (arrowheads). With 2-hour and 4-hour pulses, more double-positive cells are located medially, but not at the ventricular surface (arrowheads). (M-P) Neurog2 shows a similar pattern to that of Neurog1, but cells expressing Neurog2 and EdU are found more laterally than Neurog1+/EdU+ cells (arrowheads). Neither of the neurogenins shows robust co-localization with EdU with an 8-hour pulse in lateral locations (P, arrow). Scale bar: 50 μm. (Q-T) Cell counts for each EdU pulse time are shown for each marker. Three sections for each sample were counted and cell numbers are averaged. Each bin is 20-μm wide and bin 1 is at the surface of the third ventricle. (U-X) Cell counts for each marker at different EdU pulse times. Three sections for each sample were counted and cell numbers are averaged. Each bin is 20-μm wide and bin 1 is at the surface of the third ventricle.

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