Figure 3

Expression of various transcription factors in thalamic progenitor cells. (A,B,D-G,I,J) Confocal images of E11.5 frontal sections showing combined immunostaining of Olig3 (A,F), NeuroD1 (B,G), Olig2 (D,I), Lhx2/9 (E,J) with 0.5-hour pulse-labeled EdU. Midline is to the left. (A,F) The dashed line delineates the border of Olig3 expression, which coincides with the lateral border of EdU-expressing cells. (B,G) The arrow in (B) shows the lateral cluster of NeuroD1-expressing cells. The arrowheads in (G) show cells co-expressing NeuroD1 and EdU, which are found within the lateral cluster of NeuroD1-expressing cells. (C,H) Combined in situ hybridization for Insm1 and PH3 shows overlapping expression of these two markers (arrow). (D,I) The arrowhead and arrow point to the location of Olig2 expression in the VZ and more lateral locations, respectively. Z-projections along the indicated lines are shown in the insets in (I), indicating the co-localization of green and red signals. (E,J) The arrow in (E) shows the lateral position of Lhx2/9-expressing cells that show minimum overlap with 0.5-hour EdU. Scale bar: 100 μm.