PPL2 dopaminergic neurons in the adult brain are lineage related to the larval DL2a dopaminergic cell cluster. (A-C') DL2a NB lineage (labeled with membrane tethered GFP) was analyzed during L3 using individual optical sections. Within the NB clone, seven cells are Otd-positive (blue). Three of them (arrows) only express otd, whereas the other four express both otd and TH (red). Scale bars: 10 μm. (D) Twin-spot MARCM-labeled wild-type cell clones were induced during early L1 and analyzed in the adult brain. Scale bar: 50 μm. (E-E'') Magnified view of the PPL2 cell cluster showing two PPL2 DA neurons (arrows) labeled with membrane tethered RFP. Two additional RFP-labeled neurons (arrowheads) do not express TH and correspond to neurons in which the TH-gal4 driver is ectopically activated. Scale bars: 10 μm. DA, dopaminergic; GFP, green fluorescent protein; L, instar larval stage; MARCM, mosaic analysis with a repressible cell marker; NB, neuroblast; otd, orthodenticle; PPL, protocerebral posterior lateral; RFP, red fluorescent protein; TH, tyrosine hydroxylase.