Twin-spot MARCM lineage analysis of larval central brain dopaminergic neurons at L1 reveals clonal relationship between dopaminergic neurons. (A-E) Membrane-tethered GFP- and RFP-labeled wild-type NB clones containing TH-positive (blue) DA neurons from different DA cell clusters: DM1b cluster (A), DM2 cluster (B), DL1 cluster (C,C'), DL2a cluster (D) and DL2b cluster (E). All panels represent individual confocal optical sections (0.5 μm thick). The red asterisk in C2 identifies the DL1 DA neuron that belongs to a different NB lineage (DL1b). The yellow asterisk in C2 and C'2 identifies the same neuron in different optical sections. Scale bars: 10 μm. DA, dopaminergic; DL, dorso lateral; DM, dorso medial; GFP, green fluorescent protein; L, instar larval stage; NB, neuroblast; RFP, red fluorescent protein; TH, tyrosine hydroxylase.