RGC dendrites and putative inhibitory postsynaptic sites visualized upon expression of tdTomato in RGCs of Thy1-YFP-NL2 retina. (A) Whole-mount or en face view of a P21 YFP-NL2-expressing (green) ON RGC, co-expressing tdTomato (red). The dendritic labeling was used to perform a 'masking' function that digitally removes YFP-NL2 outside the labeled cell, as shown at high magnification in the right panels. The YFP-NL2 puncta identified using a semi-automated dotfinder program (magenta dots) are superimposed onto the image of the YFP fluorescence. (B) Whole-mount view (top) and orthogonal (bottom) projections of large-field ON RGCs at several postnatal ages.