Figure 3
YFP-NL2 colocalizes with postsynaptic and presynaptic markers of inhibitory synapses in P21 to 24 retinas. (A) Single plane confocal images of vertical slices from Thy1-YFP-NL2 retinas labeled with antibodies against the postsynaptic markers gephyrin, the γ2 subunit of GABAA receptors and glycine receptors (GlyR). (B) YFP-NL2 colocalizes with presynaptic markers of inhibitory but not excitatory synapses. Vesicular GABA and glycine transporter (VGAT) was used as a marker of inhibitory presynaptic sites, and CtBP2 as a marker of excitatory presynaptic sites. Higher magnification views (smaller panels on right) are shown for the boxed regions. For (A, B), two-dimensional cross-correlation coefficient plots of the pixel intensities in the green (YFP-NL2) and red (immunolabeling) channels, and between pixels in the green and rotated red channels, are shown in the right columns.