Figure 1

NL2 colocalizes with markers of inhibitory synapses in wild-type (WT) mice. (A, B) Single plane confocal images of P21 to P25 vertical sections of WT retina labeled with antibodies against NL2 and the inhibitory or excitatory synapse markers gephyrin, γ2 subunit of GABA_A receptors or glycine receptors (GlyR) and ribbons (carboxy-terminal binding protein 2 (CtBP2)). IPL, inner plexiform layer. Higher magnification views (smaller panels) are shown for the boxed regions in (A, B). (C) Two-dimensional cross-correlation coefficients of pixel intensities in the red and green channels are plotted to demonstrate whether the signals show non-random colocalization (peak at 0,0), or random colocalization (flat distribution). See Materials and methods for analysis. As a control for random colocalization of signals, correlation plots were obtained after the red channel was rotated 180° relative to the green (NL2) channel.