Figure 7

Ngn2 knockdown by in utero electroporation reproduces previously described phenotypes attributed to Ngn2. In utero electroporation of shRNA targeting Ngn2 reproduced several of the phenotypes we previously attributed to Ngn2. (A,B) Low magnification of the cortex at P14 electroporated with either the control shRNA (A) or the shRNA targeting Ngn2 (B) were immunostained for EGFP, and DNA was stained with Draq5. While no detectable phenotype was identified in cortices electroporated with the control shRNA (A), several phenotypes were identified in the cortices electroporated with the shRNA targeting Ngn2 (B). Arrowheads denote misplaced cells due to an inhibition of migration. Boxes identify neurons with either misoriented or multiple apical dendrites (shown in (C-J)). High magnification reveals the dedritic defects. (C-J) The neurons were immunostained with EGFP (C,G) to reveal morphology, Cux1 (D,H) to confirm laminar identity, and Draq 5 (E,I). (F,J) Merged images. (K,L) In utero electroporation also identified lateral projecting axons emerging from neurons located in the cortical plate.