A forward genetic screen with a thalamocortical axon reporter mouse yields novel neurodevelopment mutants and a distinct emx2 mutant phenotype

Table 1 Mutants found in thalamocortical development screen

Line	Mutant	Forebrain phenotype	TCA phenotype	Other phenotypes	Map interval (Chr: Mb)	Mutant gene
ND15	magoo	Smaller overall, enlarged lat. ventricles	Occasional ventral misrouting in vTel	Craniofacial, microphthalmia	19: 32.0-35.9	?
ND21	outtestine	Slightly small cortex, OBs	Normal	Short limbs, omphalocoele	12: 101.8-104.5	GMAP210 [37]
ND33	bumpy	Cobblestone surface	Fascicles to ectopia outside pia	Stunted tail	Not maintained	?
ND58	baffled	Slightly small cortex and OBs, round hippocampus	Disorganized in vTel, some stalled at CSB	Small kidneys, cleft palate, breathing defect	2: 33.8-34.8	?
ND71	fuddle	Enlarged lat.ventricles, hippocampal hypoplasia	Ventral misrouting in vTel (partial)	Iris coloboma, cleft palate	19: 20.4-37.3	?
ND87	wanderer	Small misshapen cortex and OBs	Severe ventral misrouting near DTB onto ventral surface	Kidney hypoplasia	19: 55-61.3	Emx2
ND91	sprawl	Normal	Overfasciculation, stalling	Normal	Not mapped	?

All mutants except ND91 were perinatal lethal. Megabase positions based on NCBI build 37; see text for markers. CSB, corticostriatal boundary; DTB, diencephalon-telencephalon boundary; OB, olfactory bulb; vTel, ventral telencephalon.

ISSN: 1749-8104