Figure 8From: Temporal-spatial changes in Sonic Hedgehog expression and signaling reveal different potentials of ventral mesencephalic progenitors to populate distinct ventral midbrain nucleiFate-mapped cells contribute to midbrain astrocytes. (A-D) Immunostaining for glutamine synthetase (GS) and β-gal (A,B) or GFAP and β-gal (C,D). Sections were imaged using a Zeiss Apotome setup. (A,B) β-gal-positive cells with astrocytic morphology overlap with GS. (C,D) Note that not all β-gal-positive cells with astrocytic morphology express GFAP. Filled arrowheads indicate cells that co-express GFAP; open arrowheads indicate cells that do not express GFAP; arrows highlight cells with a neuronal morphology. Scale bars: (A-B'') 10 μm; (C-D'') 40 μm.Back to article page