Skip to main content
Figure 3 | Neural Development

Figure 3

From: Temporal-spatial changes in Sonic Hedgehog expression and signaling reveal different potentials of ventral mesencephalic progenitors to populate distinct ventral midbrain nuclei

Figure 3

Changing populations of precursors are marked with Shh- and Gli1-GIFM at different stages of development. (A-N) Immunofluorescent staining for DA neurons (TH, green) and EYFP-positive fate-mapped cells (red) on E12.5 coronal sections of the mesencephalon showing the distribution of marked cells in the ventricular zone and their contribution to TH-positive DA neurons. The labeling is mosaic, since only a subset of cells is recombined in a given domain. Asterisks indicate a decreased contribution of marked cells to the medial precursor domain. Arrowheads in (A) indicate TH/EYFP-positive cells that are located more lateral than their precursor domain. The mesencephalon ventricle is outlined. v, ventral; d, dorsal. Note that there is less recombination with Gli1-GIFM (I-N) than with Shh-GIFM (A-K). Scale bar: 100 μm. (O) The representative sections shown in (A-N) are at the level of the anterior and posterior mesencephalon as indicated in the schematic. (P) Schematic of fate mapping strategy.

Back to article page

Neural Development

ISSN: 1749-8104