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Figure 2 | Neural Development

Figure 2

From: Temporal-spatial changes in Sonic Hedgehog expression and signaling reveal different potentials of ventral mesencephalic progenitors to populate distinct ventral midbrain nuclei

Figure 2

Initial domains of cells marked with Shh- or Gli1-GIFM in comparison with other ventral midbrain markers. (A-Y) In situ hybridization (A-J) and immunostainings (K-Y) on E10.5 and E12.5 coronal sections for markers of the DA precursor domain (Lmx1a, Msx1, Corin) and the RN precursor domain (Sim1, Nkx6-1). Foxa2 encompasses both precursor domains. Nkx2-2 labels a precursor domain that is thought to give rise to GABAergic interneurons. (K-Y) Shh-GIFM (K-Q') and Gli1-GIFM (R-Y'). TM was administered at the indicated time points and marked cells were analyzed at E10.5 or E12.5 with EYFP (green) and Nkx6-1 or Nkx2-2 (red) immunostaining. The Lmx1a- expressing (yellow or orange dashed line) and the Foxa2-expressing (blue dashed lines) cells are outlined in some sections. Arrows indicate double-labeled cells, arrowheads in X' and Y' indicate fate-mapped cells in the Nkx6-1 negative medial domain. The medial-lateral extent of the initial fate-mapped domains reflects the endogenous gene expression around the time of TM administration (compare with Figure 1). Note that the labeling is mosaic, since only a subset of cells is recombined in a given domain. Panels (L'-Y') are higher magnifications of the areas indicated with the dashed box in (L-Y). (Z) Distribution of cells fate-mapped at the indicated time points. The summary is based on the immunostainings and in situ hybridizations at E10.5 and E12.5. To assess the distribution of the fate-mapped cells, at least three sections were analyzed for each TM time point at E10.5 and E12.5. To determine the expression domains of the specific transcription factors, sections from at least three embryos were analyzed. Scale bars: 100 μm.

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