Presynaptic components localize to the correct subcellular compartment in unc-6 and unc-40 mutant animals. (A,B) Wild type, (C,D) unc-6, (E,F) unc-40, and (G,H) cfi-1; unc-42 animals are labeled with the presynaptic vesicle marker mCherry::rab-3 expressed in PHB neurons. Presynaptic specializations are unaltered in unc-6, unc-40, and cfi-1; unc-42 mutant animals and localize to the distal region of the PHB axon within the preanal ganglion, where PHB synapses normally form (boxed in white). Yellow scale bar: 2 μm. (I) Quantification of mCherry::RAB-3 fluorescence intensity using NIH ImageJ indicates no significant difference among wild-type, unc-6 and unc-40 animals. Wild-type n = 32, unc-6 n = 47, and unc-40 n = 36 animals. NS, not significant, Kruskal-Wallis test. (J) Quantification of mCherry::RAB-3 fluorescence intensity using NIH ImageJ indicates no significant different among wild-type and cfi-1; unc-42 animals. Wild-type n = 45, and cfi-1; unc-42 n = 45 animals. NS, not significant, u-test.