Figure 9

Loa/Loa dynein can still bind its regulatory partners. (A) GST-NudE pull-down assay from wild-type and Loa/Loa brain lysate. Input is 30% of total. Immunoblot reveals that protein is not pulled down by beads alone (BA), but similar amounts of both wild-type (+/+) and Loa/Loa (L/L) dynein (as shown by immunoblotting for dynein heavy chain and dynein intermediate chain) along with LIS1, are pulled down by GST-NudE. HC, heavy chain; IC, intermediate chain. (B) GST-CC1 pull-down assay from wild-type and Loa/Loa brain lysate. Input is 30% of total. Immunoblot reveals that dynein is not pulled down by beads alone (BA), but similar amounts of both wild-type (+/+) and Loa/Loa (L/L) dynein (as shown by immunoblotting for dynein heavy chain and dynein intermediate chain) are pulled down by GST-CC1. A higher exposure blot reveals the GST-CC1 band below the IC band. (C) Graph depicting the average percentage of wild-type or Loa/Loa dynein heavy chain (± standard deviation) that is pulled into the pellet by either GST-NudE or GST-CC1 (n = 3 experiments per genotype).