Figure 5
From: Neuronal migration defects in the Loa dynein mutant mouse
Live cell imaging of radial migration in the intermediate zone. Mouse brains were sectioned and incubated with Oregon Green BAPTA-1 488 AM, and the labeled bipolar cells were imaged within the IZ. (A) Kymographs of somal translocation within the neurons of the wild-type and Loa/Loa mouse brains. Time is shown in minutes. Scale bar = 10 μm. (B) Tracings of cell body position for six representative wild-type neurons and six representative Loa/Loa neurons show a slower progression of somal movement in the Loa/Loa neurons. (C) Graph depicting the average rates of leading process outgrowth and somal translocation (± standard deviation) for wild-type and Loa/Loa neurons (P < 0.0001 for somal translocation; n = 12 cells from at least three brains per genotype followed for at least 110 minutes).