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Figure 3 | Neural Development

Figure 3

From: Zac1 plays a key role in the development of specific neuronal subsets in the mouse cerebellum

Figure 3

Zac1 marks a subset of late-born cerebellar Golgi cells. (A-J) Double immunofluorescence staining of P7 cerebella for Zac1 and calretinin (A-C), mGluR1α (D-F), parvalbumin (G-I) and NG (J; first immunoperoxidase stained with DAB for Zac1 (brown), then fluorescence stained for NG (red)). Arrowheads in (J) mark Purkinje cells. (K,L) Primary cultures of E18 mouse cerebella (21 days in vitro) co-labeled with anti-Zac1 (red) and anti-NG (green). Arrows in (K) mark Zac1+ Golgi cells that co-express NG. (M,N) Birthdating of NG+ (M) and Zac1+ (N) Golgi cells, showing cells labeled with BrdU injections at E12 and analyzed at P20 (M) and BrdU injections at E14 and analyzed at P5 (N). (O) Quantification of the distribution of BrdU-immunostained Golgi cell nuclei in the P5 (for Zac1) and P20 (for NG) cerebellar cortex after exposure to BrdU at different stages during embryonic development (each stage, N = 4 pups, counted from sagittal sections; error bars represent standard deviation). Scale bars: 25 μm (H,J).

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