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Figure 1 | Neural Development

Figure 1

From: Coordinated trafficking of synaptic vesicle and active zone proteins prior to synapse formation

Figure 1

STVs and PTVs move together. (A) Kymographs of an axon segment showing the movements of PTVs (GFP-bassoon; green) and STVs (synaptophysin-mcherry; magenta) in the same axon. Yellow lines underline the moving STVs and PTVs. Bottom panel: overlay of STV and PTV fluorescence. On the ordinate axis, one pixel corresponds to 10 s. On the abscissa, the scale bar corresponds to 10 μm. (B) Simulations of model axons were performed by randomizing initial positions of vesicles while maintaining movement and pausing characteristics of the original imaged vesicles. Diagrams illustrate kymographs of three simulations of model axons. Movements of two vesicles are shown in each model axon. (C) Plot showing the percentage of PTVs that move with STVs (green) and STVs that move with PTVs (magenta). PTV and STV simulations (light green and light magenta, respectively) correspond to the predicted values from the simulations. (D) Quantification of the percentage of time that PTVs and STVs spent together. Both the percentage of vesicles that moved together and the time spent together were substantially larger in the data than is predicted by chance (simulations).

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