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Figure 8 | Neural Development

Figure 8

From: Rostral growth of commissural axons requires the cell adhesion molecule MDGA2

Figure 8

Down-regulation of MDGA2 on the contralateral side causes phenotypes similar to those seen in ipsilateral knockdowns. Embryos were injected with either of the two non-overlapping long dsRNA fragments covering the sequences -15 to 1,248 and 1,975 to 2,746, respectively. Following contralateral electroporation, embryos treated with either fragment did show severe defects in commissural axon growth. (A,C) While the table (A) gives the fluorescent intensities measured at different locations in control and RNAi-treated embryos (for details see Figure 4 and Materials and methods), the histogram (C) depicts the normalized fluorescent intensities in percentage of the control. In analogy to the results seen with ipsilateral electroporations of MDGA2 dsRNA (Figure 4), rostral turning of commissural axons after midline crossing in contralateral RNAi knockdown embryos is strongly reduced compared to control embryos. (B) Representative confocal pictures of open-book preparations of embryos after MDGA2 knockdown are shown. Most post-crossing commissural axons stalled at the floor-plate exit site and did not grow along the longitudinal axis. FP, floor-plate. Error bars given as standard errors (SEM). Scale bar: 100 μm.

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