Figure 7
From: EphA4 expression promotes network activity and spine maturation in cortical neuronal cultures
Characteristics of dendritic spines in control- and EphA4-transfected neurons at 14 DIV. (A,B) Dendritic shafts (horizontal) with protruding dendritic spines in control (A) and EphA4 (B) transfected neurons at 14 DIV. Arrowheads point to mature spines while arrows mark immature spines. (C) Neurons overexpressing EphA4 have a significantly higher proportion of mature dendritic spines compared to control neurons (**P < 0.01 as determined using ANOVA). (D-G) Dendritic spines of neurons transfected with actin-GFP and control (D,E) or EphA4 (F,G) and stained for PSD-95 (alone in (D,F)). Arrowheads point to spines with PSD-95 puncta while arrows mark spines without associated PSD-95. (H) A slight increase in the proportion of spines containing a PSD-95 puncta is seen in neurons overexpressing EphA4 compared to control neurons.