Figure 9

Immunohistochemistry with antibody specific against P. clarkii 5-HT _2β receptor reveals protein expression in some cell soma clusters (n = 14 brains). (A) Low magnification images show the overall staining pattern seen from the dorsal side. The large arrow points to the paired medial giant neurons, small arrows indicate the locations of clusters 6, 16, and 17, and arrowheads point to the MPZ and LPZ. (B-F) Higher magnification images of staining in the medial giant cells and their fibers (B, arrows), cluster 6 (B, arrowheads), cluster 17 (C), cluster 16 (D), the LPZ of cluster 10 (E) and the MPZ of cluster 9 (F). Scale bars: 200 μm (A); 50 μm in the other panels. AL, accessory lobe; cl, cluster; OL, olfactory lobe.