Figure 6
Immunohistochemistry with antibody specific against P. clarkii 5-HT 1α receptor reveals protein expression in cell soma clusters and synaptic neuropil regions (n = 11). (A, H) Low magnification images show the overall staining pattern in ventral (A) and dorsal (H) views of the brain. (C-Di, F-Gi, K) Higher magnification images of staining in clusters 6 (C), 9 (G, Gi), 10 (F), 11 (K) and 16 (D, Di) are also shown. (B, E, I-Ji) Intensive 5-HT1α protein is also found in neuropils, such as in the anterior (AMPN) and posterior median protocerebral neuropils (PMPN) (B), accessory lobe (AL; (E) with two AL spherical glomeruli circled), olfactory lobe (OL; I, Ii), and protocerebral bridge (PCB; arrows in (J, Ji)). Scale bars: 400 μm (A, H); 100 μm (J); 50 μm (A, C, G, I, Ji); 20 μm (C, F); 10 μm for all other panels.