Figure 5
Whole mount in situ hybridization for P. clarkii 5-HT 2β (n = 5). (A) Low magnification ventral view of the brain. (B) Low magnification dorsal view of the brain. (C-F) More highly magnified views of selected cell clusters (cl). Intense 5-HT2β message (purple to black) is found in soma clusters 9 (C), 10 (D), 11 (B, arrows; E) and 17 (F) with antisense riboprobe. (G, H) Combined with immunofluoresence for GS (H, red), it is clear that cells in the niche and streams are not labeled (G). The red dotted line is placed just below the outline of the niche and streams that are visualized in (H). Arrows in (G, H) mark the same point where the stream inserts into cluster 10. (I) The sense probe indicated no detectable signal. Scale bars: 300 μm (A, B); 100 μm (C-F); 200 μm (G-H); 500 μm (I). AL, accessory lobe.