Figure 12
Immunocytochemical labeling for the serotonin transporter (n = 9 brains). (A-F) SERT is green in (A-D) and blue in (E-F). Structures with intense labeling for SERT include the cytoplasm of neurons in clusters 9 and 10 (A, C-F), the medial giant neurons (A, arrowhead; B, arrows), and the neurogenic niche and migratory streams (E-F). A lower level of immunoreactivity is seen in the LPZ (A, arrow) relative to surrounding cells in cluster 10, similar to the distribution of 5-HT1α mRNA (Figure 7). (C) SERT labeling of cells in cluster 10. (D) DAPI (4',6-diamidino-2-phenylindole, cyan in (D)) labeling of DNA merged with SERT labeling shown in (C). (E) SERT labeling of clusters 9 and 10, and the neurogenic niche and streams (arrowheads). (F) BrdU labeling (red) merged with SERT labeling shown in (E). Scale bars: 200 μm (A, B, E, F); 20 μm (C, D). AL, accessory lobe; cl, cluster; OL, olfactory lobe.