Figure 10
Double immunofluoresence for 5-HT 2β (green)/glutamine synthetase (blue) (n = 4 brains) or 5-HT 2β (green)/BrdU (red) (n = 4 brains) reveals the expression of 5-HT 2β protein in S phase cells in the distal streams near the proliferation zones, and in the proliferation zones themselves. (A, B) Cells in the neurogenic niche (A) and proximal and medial parts of the migratory streams (B, arrow indicates a GS-positive cell in the proximal part of the stream) do not label for the receptor. (C-Fi) Some cells in the distal end of the stream near the proliferation zones (arrows in (C, Ci, E, Ei)), as well as cells in the proliferation zones of cluster 10 (arrows in (D, Di, F, Fi); insets show more highly magnified proliferation zones) show cytoplasmic labeling for the receptor. The left panels (C, D, E, F) show labeling for the 5-HT2β receptor; the companion panels on the right (Ci, Di, Ei, Fi) are composites showing receptor immunoreactivity merged with other antibody labels. Scale bars: 50 μm (F, Fi); 10 μm ((B) and insets); 20 μm in the other panels.