EBF factors drive expression of multiple classes of target genes governing neuronal development

Table 1 Candidate targets of EBF activity

Gene name	Function	FC¹ (microarray)	FC² (RT-QPCR)	GenBank
pcdh8	Transmembrane protein	66	93^a	BC074360
nr2f2	Nuclear receptor TF	47	ND	BC078057
wnt3a	Wnt signaling ligand	45	ND	L07538
peripherin	Type III intermediate filament	37	32^a	BC056020
greb1	Estrogen-regulated gene	32	21^b	BC043838
hoxd10	Homeodomain TF	31	ND	BC061944
nf-m	Type IV intermediate filament	28	29^b	BC078128
kcnk5	K+ ion channel	27	6^b	BC084931
nscl-1	bHLH TF	26	7^b	BC084434
neurod	bHLH TF	26	164^b	BC072996
en-2	Homeodomain TF	24	ND	X62974
aml1	Runt-related TF	22	24^a	BC057739
activin beta b	TGF-beta superfamily member	21	154^b	S61773
emx1	Homeodomain TF	16	15^b	BC077629

The genes chosen for analysis are shown with their known functional roles. FC¹ refers to the average fold change in expression by microarray analysis after DEX treatment for 4.5 hours compared to control, in four replicate experiments. FC² refers to the fold change in expression after DEX treatment for 3 hours compared to control, as detected by RT-QPCR using independent samples (see Figure 6 and Additional file 6 for details). ^aPerformed three replicate experiments; ^bperformed once. FC, fold change; ND, not determined; TF, transcription factor.

ISSN: 1749-8104