Figure 1
From: EBF factors drive expression of multiple classes of target genes governing neuronal development
Candidate target genes upregulated by overexpression of EBF2 or EBF3. hGR-XEBF2 or hGR-XEBF3 mRNA was injected into one cell of two-cell stage embryos, followed by DEX treatment from the late gastrula stage (stage 11/11.5) to the neurula stage (stage 14/15). β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-T) The expression levels of pcdh8 (A,B), peripherin (C,D), greb1 (E,F), nf-m (G,H), kcnk5 (I,J), nscl-1 (K,L), neurod (M,N), aml1 (O,P), activin beta b (Q,R), and emx1 (S,T) are strongly upregulated by EBF2 and EBF3 (brackets). Panels (A-R) show dorsal views, while (S,T) show anterior views.