Figure 2

Endogenous Bazooka protein expression at third instar. (A-E) Bazooka expression visualized with a rabbit anti-Bazooka monoclonal antibody [39] and shown at varying distances into the brain (distance indicated in lower right in each panel) from anterior to posterior in 2 μm sections. Secondary axon tracts (SATs) are labeled according to published nomenclature [24]. (A) Magnified lineage shows Bazooka expression in the neuroblast (arrowhead), ganglion mother cells (GMCs) and newborn neurons (double arrowheads), and older neurons (arrow). (E) Note the apical accumulation of Bazooka protein in the apical mushroom body (MB) neuroblast and neuroepithelia (lateral is shown to the left and medial is to the right, with midline indicated by a dotted line). (F) A cartoon depiction of Bazooka localization (green) in the apical neuroblast, newly born neurons and to a lesser extent late-born neurons, and in the bundled axon tracts. Scale bar: 25 μm