Effects of perturbing Diap1, p35 and Dark on RP2 death. (A) UAS-Diap1 animals showed normal patterns of GFP-labeled neurons at 8 h-APF (A1) and RP2 death and aCC survival at 20 h-APF (A2). (A3) RP2 death was likewise unimpaired in thSL mutants, in which Diap1 inactivation by Reaper is suppressed. (B) Expression of p35 via UAS-p35 blocked the death of RP2s, which were still present at 24 h-APF. (C) In a darkCD4/darkCD4 animal (homozygous for a dark hypomorphic allele), RP2s were still present at 24 h-APF. All images are from mid-abdominal neuromeres. Scale bars: 20 μm.