Inactivation of Rbpj results in reduced cell proliferation and increased apoptosis in E11.5 DRG . Wild-type (WT) and Rbpj CKO embryos were pulse labeled with BrdU 2 hours prior to fixation. (A-D) BrdU incorporation in Rbpj-deficient DRG is comparable to that in wild-type controls at E10.5 (A,B), but greatly reduced at E11.5 (C,D). (E-F) TUNEL staining of transverse sections through DRG (outlined) shows that there is no difference in the rate of apoptosis between wild-type and CKO DRG at E10.5 (E,F), but the number of TUNEL-labeled cells is significantly increased in Rbpj-deficient DRG relative to controls at E11.5 (G,H). Scale bar: 100 μm. (I,J) Statistical analysis of BrdU+ (I) or TUNEL+ cells (J) in wild-type and Rbpj CKO DRG. Error bars represent standard error of the mean; *P < 0.01.