Figure 4

Increased TrkC⁺neurons in Rbpj -deficient DRG at E10.5. (A-D,F,G) Transverse sections through DRG of wild-type (WT) and Rbpj CKO hybridized with the indicated probes. Brn3a (A,B), SCG10 (C,D) and NeuroD1 (F,G) expression levels are all enhanced in Rbpj-deficient DRG compared with wild-type controls. High magnification views of the areas delineated by black rectangles in (C,D,F,G) are shown at the bottom of each panel. (E) Comparison of the number of SCG10⁺ or NeuroD1⁺ cells in the DRG between wild-type and Rbpj CKO mice at E10.5. Error bars represent standard error of the mean; *P < 0.05. (H-M) The expression of TrkA (H,I) and TrkB (J,K) does not differ notably between the two groups, but TrkC expression (L,M) is greatly up-regulated in Rbpj-deficient DRG compared with wild-type controls. Arrows point to the DRG. VH, ventral horn. Scale bars: 100 μm.