Radial glial cells located in defined walls of the lateral ventricle produce distinct granule neuron subtypes. (A-C) Representative immunostainings for CR expression in the GCL of animals electroporated in the lateral (A), dorsal (B) or septal (C) walls of the LV. For all groups, a low magnification overview (left panel) as well as a higher magnification of the underlined region (right panel) are presented. DAPI (blue) was used as a nuclear counterstain. (D) Quantification of the percentage of newborn granule cells (GFP+) expressing CR that derived from the lateral (red), dorsal (gray) or septal (blue) LV walls. Error bars represent standard error of the mean. *P < 0.05 as determined by unpaired t-test. Scale bars: 50 μm and 10 μm in the left and right panels, respectively. CR, calretinin; EPO, electroporation.