Glutamatergic phenotype of Tbr2- and Tbr1-positive juxtaglomerular OB neurons. (A,D) Tbr2 and Tbr1 are found in VGluT-positive cells, as revealed by combining in situ hybridization for VGluT1/2 (here imported into the red channel) and immunostaining in the green channel. Images are taken from the GL. (B,C,E,F) Tbr2 and Tbr1 do not co-localize with GAD67-GFP (B,E) nor GAD65 (C,F), confirming the non-GABAergic identity of these cell populations. Images are from the GL. (G-L) Tbr2 and Tbr1 do not co-localize with known periglomerular cell markers (TH, CR, CB or parvalbumin) in the GCL. Scale bars: 50 μm in (A-D); 20 μm in (G-R).