Maturation-dependent reduction in excitatory postsynaptic current frequency in Dgcr8+/- mPFC. (A,B) Representative excitatory postsynaptic current (EPSC) recordings from L5 pyramidal neurons from (A) WT and (B) Dgcr8+/- mice. Scale bar = 20 pA, 200 ms. (C) Mean EPSC responses (average of >50 isolated events) from individual WT (black) and Dgcr8+/- (grey) neurons superimposed on the same scale illustrate no changes in EPSC amplitude or kinetics. (D,E) Summary of EPSC parameters from WT (n = 15) and Dgcr8+/- (n = 18) neurons reveals a maturation-dependent reduction in EPSC frequency from between P16 and P21 to between P25 and P30. (F,G) Inhibitory postsynaptic current (IPSC) recordings from (F) WT and (G) Dgcr8+/- pyramidal neurons. Scale bar = 50 pA by 500 ms. (H) Mean IPSC responses (average of >50 isolated events) from individual WT (black) and Dgcr8+/- (grey) neurons; (I,J) summary graphs of amplitudes and frequencies demonstrate no changes in inhibitory synaptic transmission at these periods. Bars represent mean ± standard error; *P < 0.01.