Reduced miRNA expression in Dgcr8+/- mice. Dgcr8 mRNA and miRNA expression in the prefrontal cortex of WT (n = 5) and Dgcr8+/- (n = 5) animals at postnatal day (P)5 and P25. (A) Quantitative PCR showing a significant reduction in Dgcr8 mRNA levels in the heterozygous animal at P25 but not at P5. (B,C) Quantitative PCR of a selected panel of miRNAs showing significant reductions of specific miRNAs at P25 but not at P5. Values are shown as relative expression compared to WT. Bars represent mean ± standard error; *P < 0.01.