Glial processes are associated with wisping axons and do not form inhibitory barriers. Axons were labeled with 3A10 (B, D) or the axon tracer (E) and are shown in magenta. Glia were labeled with anti-GFAP (green), and nuclei are blue (A-D). (A) Cross-section through intact spinal cord. GFAP labels EG and astrocytes and all of their processes. (B) Longitudinal section through the SCI of a wisping stage regenerate. Astrocytes do not become hypertrophic (compare with (A)). (C) Section adjacent to (B) treated only with secondary antibodies; primary antibodies were omitted. (D) Longitudinal section through the SCI of a wisping stage regenerate. (D') Enlargement of dotted box in (D). GFAP+ processes (arrows) are associated with wisping axons and do not form a glia limitans. Only a few weakly GFAP+ cells (arrowhead) are in the injury site. (E) Single confocal plane of a longitudinal thick section of a growth beyond stage regenerate. GFAP+ processes run parallel to axons across the injury site. D, dorsal; V, ventral; R, rostral; C, caudal. Scale bars: 200 μm (A-D; (B) and (C) are the same scale; (D) and (E) are the same scale); 50 μm (D').