Figure 6From: Cortico-cerebral histogenesis in the opossum Monodelphis domestica: generation of a hexalaminar neocortex in the absence of a basal proliferative compartmentAnalysis of the apical progenitor compartment. (A) Pax6 immunoprofiling of a selection of coronal sections from P4 to P15 opossum telencephalons. (B) Linear densities of Pax6+ cells sampled in (A). (C) EGFP/β-tubulin immunoprofiling of opossum cerebral cortex, dissected out at P10, acutely electroporated with a pTα1-EGFP plasmid and kept in in vitro culture for 48 h. Magnifications of the boxed area in (Ca) are shown in (Cb-b"). Arrowheads in (Cb-b") point to pial processes of electroporated cells, immunoreactive for EGFP, but not for β-tubulin. Abbreviations: i-cp, inner cortical plate; o-cp, outer cortical plate. Scale bars: 400 μm in (A); 100 μm in (C).Back to article page