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Figure 5 | Neural Development

Figure 5

From: Cortico-cerebral histogenesis in the opossum Monodelphis domestica: generation of a hexalaminar neocortex in the absence of a basal proliferative compartment

Figure 5

Origin and fate of Tbr2+ cells. (A, B) Confocal Tbr2/enhanced green fluorescent protein (EGFP) (Aa-b") and Tbr2/β-tubulin (Ba, b) immunoprofiling of opossum cerebral cortex, dissected out at P10, acutely electroporated with a pTα1-EGFP plasmid and kept in in vitro culture for 48 h. Magnifications of boxed areas in (Aa) and (Ba) are shown in (Ab-b") and (Bb), respectively. (Bc-h) Colocalization of Tbr2 and β-tubulin on acutely dissociated cells from P10 opossum cortex. (C) Confocal Tbr2/BrdU immunoprofiling of neocortical coronal sections from opossum pups, pulsed with BrdU at P6 and sacrificed after different times: 1 h, 12 h, 24 h, 48 h and 72 h. Arrowheads in (Cc-e) point to cells immunoreactive for both BrdU and Tbr2. (D) Relative radial distribution of BrdU+ cells sampled in (C) compared to the Tbr2+ belt. (E) Time course of percentages of Tbr2+cells sampled in (C) also immunoreactive for BrdU. Abbreviations: cp, cortical plate; iz, intermediate zone; mz, marginal zone; sp, subplate; vz, ventricular zone. Scale bars: 100 μm in (A-C); 20 μm in (Ab, Bb, c).

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Neural Development

ISSN: 1749-8104