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Figure 3 | Neural Development

Figure 3

From: Cortico-cerebral histogenesis in the opossum Monodelphis domestica: generation of a hexalaminar neocortex in the absence of a basal proliferative compartment

Figure 3

Bromodeoxyuridine birthdating of opossum neocortical neurons. (A) BrdU immunoprofiling of mid-frontal neocortical sections from opossums injected with a single pulse of BrdU at the ages of P1, P4, P6, P8, P10, P12, P14, P16 and P18, and fixed at P30. Arrowheads point to grey matter BrdU+ cells. (B) Diagramatic representation of BrdU+ cells sampled in (A): the cortical wall is divided into 20 equally spaced bins, numbered from ventricular to marginal; radial extension of cortical laminae is indicated by white/grey shading; plots representing percentages of BrdU+ cells falling into each bin, for each injection time. (C-H) Colocalization of layer-specific markers, Tle4 (C), Cux1 (D), Calb2 (E-H), with BrdU injected at P4 (C), P12 (D) and P7 (E-H), respectively. Solid arrowheads in (E-H) point to Calb2+/BrdU+ cells; empty arrowheads in (F, H) point to Calb2-/BrdU+ cells. Abbreviations: I, II, III, IV, V, VI refer to cortical layers; e, ependyma; se, subependymal zone; wm, white matter. Scale bars: 100 μm in (A); 40 μm in (C-H).

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