Figure 3
From: Nav2 is necessary for cranial nerve development and blood pressure regulation
Nav2-/-/ Brn3a-lacZ+/- mice show a reduction in lacZ staining of sensory nerves compared to their Nav2+/+/ Brn3a-lacZ+/- littermates. (A) Nav2 wild-type (WT) and (B) homozygote mutant (HOM) littermates in a Brn3a-lacZ+/- background shown at the same developmental stage (E13.5). (C, D) Specifically, there appear to be fewer sensory neurons in the mesencephalic tract (mes) region in the Nav2-/-/Brn3a-lacZ+/- embryo (D) (E13.5) compared to the wild-type (WT) littermate (C) (E13.5). (E, F) A reduction in spinal nerve staining is also observed in the Nav2-/-/Brn3a-lacZ+/- embryo at E15.5 (compare (F) to the wild-type in (E)). The results shown are representative of nine wild-type and nine Nav2-/-embryos examined at E12.5 to 13.5, and two wild-type and three Nav2-/-embryos at E15.5. Scale bar: 200 μm. fl, forelimb; st, stomach.