The unfulfilled gene is required for the development of mushroom body neuropil in Drosophila

Table 1 Mushroom body phenotypes in unf mutants and rescue animals.

Row	Genotype	All lobes present adult/larvae (%)	All lobes missing adult/larvae (%)	Dorsal lobes missing adult/larvae (%)	Missing a dorsal or medial lobe adult/larvae (%)	n adult/larvae
	Controls
1	w ¹¹¹⁸	100	0	0	0	10
2	unf^X1/+	100/100	0/0	0/0	0/0	12/8
3	unf^Z0001/+	100/100	0/0	0/0	0/0	7/15
4	Df/CyOGFP	100/100	0/0	0/0	0/0	8/8
5	UAS-GFP;;OK107	100	0	0	0	10
6	unf^X1, UAS-GFP/CyOGFP;;OK107	100	0	0	0	15
7	unf^Z0001, UAS-GFP/CyOGFP;;OK107	90	0	10	0	10
	*unf* mutants and gene knock down
8	unf^X1,UAS-GFP/Df;;OK107	0/0	100*/0	0/90*	0/10	15/11
9	unf^X1,UAS-GFP/unf^X1;;OK107	0	100*	0	0	7
10	unf^Z0001/Df, UAS-GFP;;OK107	0/0	0/0	100/100	0/0	13/6
11	unf^Z0001/unf^Z0001,UAS-GFP;;OK107	37.5	0	37.5	25	8
12	unf^MB05909/Df, UAS-GFP;;OK107	100	0	0	0	14
13	+/Df;UAS-unf^RNAi;OK107	50	0	40	10	10
14	unf^X1,UAS-GFP/unf^Z0001;;OK107	0	13	47	40	15
	*unf* transgenic rescues
15_a	unf^X1,UAS-GFP/Df;UAS-unf^rsqF/+;OK107	100/83	0/0	0/0	0/17	11/6
16_b	unf^Z0001/Df, UAS-GFP/UAS-unf^rsqF/+;OK107	100*	0	0	0	8
17_c	unf^X1,UAS-GFP/Df;UAS-unf^rsqC/+;OK107	86*	0	0	14	7
	*unf* rescue controls
18_a	unf^X1,UAS-GFP/Df;TM3Sb/+;OK107	0	100	0	0	6
19_a	unf^X1,UAS-GFP/CyO;UAS-unf^rsqF/+;OK107	100	0	0	0	9
20_a	unf^X1,UAS-GFP/CyO;TM3Sb/+;OK107	100	0	0	0	4
21_a	Df/CyOGFP;UAS-unf^rsqF/+;OK107	100	0	0	0	5
22_a	Df/CyOGFP;TM3Sb/+;OK107	100	0	0	0	7
23_b	unf^Z0001/Df, UAS-GFP;TM3Sb/+;OK107	0	0	100	0	7
24_c	unf^X1,UAS-GFP/Df;TM3Sb/+;OK107	0	100	0	0	9
25	unf^X1,UAS-GFP/Df;UAS-lacZ/+;OK107	0	100	0	0	9
26	UAS-GFP;UAS-unf^rsqF/+;OK107	100	0	0	0	5

Data are presented as percentages of whole brains that exhibit the phenotype. Control and mutant animals are 0- to 2-day adults, whereas rescue and rescue control animals are 72- to 96-hour pupae. All larvae are third instars. Single entries are adult or late pupae. Rescues and the corresponding control siblings are noted by matching subscript letters in rows. Asterisks indicate P-values of < 0.01 from the Fisher exact test. Abbreviations: Df, Df(2R)ED2426; UAS-GFP, UAS-mCD8GFP; OK107, OK107-GAL4.

ISSN: 1749-8104